General methods in yeast genetics specify using yeast extract-peptone-dextrose (YPD) medium for cultivating Saccharomyces cerevisiae and other yeasts. Yeasts grow well on a minimal medium containing only dextrose and salts. The addition of protein and yeast cell extract hydrolysates allows faster growth so that during exponential or log-phase growth, the cells divide every 90 minutes.
Peptone acts as a source of carbon, nitrogen, vitamins and minerals
Yeast extract supplies B-complex vitamins, which stimulate bacterial growth
Dextrose is the carbohydrate source
pH (6.5% solution, 25°C): 6.5 ± 0.2
Specifications
Beige
<30°C
For maintaining and propagating Yeasts in molecular microbiology procedures